- We use Cell Signaling Technology #9718 gamma H2AX antibody, but I believe your antibody is also good. - Yes, we are doing western blot - Either 4-15% or 4-20% TGX gels from Bio-Rad are used

Histone H2AX, Histone H2A.X, H2AX_HUMAN. UniProt Code History. P16104, Q4ZGJ7, Q6IAS5. Gene Synonyms. H2AX, H2a.x, H2afx, H2A/X. Supplier also known as. CST, Cell Signalling, New England Biolabs

Haematologica. 2010 Dec;95(12):1996-2004. doi: 10.3324/haematol.2010.026492. Epub 2010 Sep 7. Delta-tocotrienol protects mouse and human hematopoietic progenitors from gamma-irradiation through extracellular signal-regulated kinase/mammalian target of rapamycin signaling. 3 reviews.

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Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb. 2008-09-04 H2A.X is required for checkpoint-mediated cell cycle arrest and DNA repair following double-stranded DNA breaks (1). DNA damage, caused by ionizing radiation, UV-light, or radiomimetic agents, results in rapid phosphorylation of H2A.X at Ser139 by PI3K-like kinases, including ATM, ATR, and DNA-PK (2,3). 2010-02-04 H2A.X is phosphorylated at Ser139 by DNA-PK in response to cell death receptor activation, c-Jun N-terminal Kinase (JNK1) in response to UV-A irradiation, and p38 MAPK in response to serum starvation (5-8). H2A.X is constitutively phosphorylated on Tyr142 in undamaged cells by WSTF (Williams-Beuren syndrome transcription factor) (9,10).

Healthy Persons. En djupare förståelse för vad som sker på cellnivå och molekylnivå i prostata- 18 Vi observerade en tydlig ökning av strålbehandlings-inducerad γ-H2AX foci i vi inte utesluta att kastration också kan påverka andra signalvägar än DNA-PK. ”Conclusion: Cell phone RF exposure induced significant hormonal and structural that the signal causes transient genetic instability in glioma derived cells and on 53BP1/gamma-H2AX foci persisted up to 72 h following exposure of cells,  ground state, accompanied by gamma radiation of 1460 keV energy in ionization of the water molecules in the cell (Cottingh and Greenwood, 2001).

1. Haematologica. 2010 Dec;95(12):1996-2004. doi: 10.3324/haematol.2010.026492. Epub 2010 Sep 7. Delta-tocotrienol protects mouse and human hematopoietic progenitors from gamma-irradiation through extracellular signal-regulated kinase/mammalian target of rapamycin signaling.

This phosphorylation event is critical for sustained recruitment of other proteins to repair the break. Histone H2AX, Histone H2A.X, H2AX_HUMAN. UniProt Code History.

Here we show that hyperthermia, an effective radiosensitizer, can induce several steps associated with IR signaling in cells. Hyperthermia induces gamma-H2AX foci formation similar to foci formed in response to IR exposure, and heat-induced gamma-H2AX foci formation is dependent on ATM but independent of heat shock protein 70 expression.

Epub 2010 Sep 7. Delta-tocotrienol protects mouse and human hematopoietic progenitors from gamma-irradiation through extracellular signal-regulated kinase/mammalian target of rapamycin signaling. Anti Ub H2ax K119, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more Rabbit recombinant monoclonal gamma H2A.X (phospho S139) antibody [EP854(2)Y] conjugated to Alexa Fluor® 647. Validated in ICC/IF and tested in Human.

Each gamma-H2AX antibody is fully covered by Novus’ Guarantee+ to give you peace of mind when doing research. Choose the anti-gamma H2AX antibody best suited for your research needs by 2017-02-03 2/14/2021 Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb (#9718) Datasheet Without Images Cell Signaling Technology https://www.cellsignal.com/datasheet.jsp?productId=9718&images=0&protocol=0 1/2 Pro d u ct U sa g e In fo rma ti o n A pplic at ion Dilut ion Wes t ern B lot t ing 1: 1000 I mmunohis t oc hemis t ry (P araf f in) 1: 480 2013-11-01 Within minutes of the induction of DNA double-strand breaks in somatic cells, histone H2AX becomes phosphorylated at serine 139 and forms gamma-H2AX foci at the sites of damage. These foci then play a role in recruiting DNA repair and damage-response factors and changing chromatin structure to accurately repair the damaged DNA. These gamma-H2AX foci appear in response to irradiation and 2009-06-15 2021-03-07 Attenuation of genotoxicity under adhesion-restrictive conditions through modulation of p53, gamma H2AX and nuclear DNA organization. Strasberg Rieber M(1), Viola-Rhenals M, Rieber M. Author information: (1)IVIC, Tumor Cell Biology Laboratory, CMBC, Apartado 21827, Caracas, Venezuela. mrieber@pasteur.ivic.ve A431 cell nucleus (DAPI signal) showing three gamma-H2AX foci structures. Primary anitibody H2A.X (phospho S139) antibody [3F2], ab22551, 100ug.
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Adriamycin or CFM-4.16 treatments inhibited cell growth and caused elevated CARP-1 and gamma H2AX in human breast (HBC) and cervical cancer (HeLa) cells. 2013-11-01 · H2AX is a member of the histone protein H2A family; the other two members are H2A1-H2A2 and H2AZ . γH2AX foci formation at DSB sites occurs rapidly (within minutes) and is highly conserved from yeast to humans . γH2AX can recruit other DSB signaling and repair factors such as MDC1, 53BP1 and the MRN complex to the damage site, forming nuclear ionizing radiation-induced foci (IRIF) , , . Gamma H2AX (gammaH2AX) is the phosphorylated version of histone H2AX and is a marker for double-stranded breaks (DSBs) caused by DNA damage (1-4).

Phosphorylation of H2AX plays a key role in DDR and is required for the assembly of DNA repair proteins at the sites containing damaged chromatin as well as for activation of checkpoints proteins which arrest the cell cycle progression. Immunohistochemical analysis of paraffin-embedded HT-29 cell pellets, control (left) or UV-treated (right), using Phospho-Chk2 (Thr68) (C13C1) Rabbit mAb. Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb. 2008-09-04 H2A.X is required for checkpoint-mediated cell cycle arrest and DNA repair following double-stranded DNA breaks (1).
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Lyse cells by adding 1X SDS sample buffer (100 µl per well of 6-well plate or 500 µl for a 10 cm diameter plate). Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity).

Which aspects of the DDR are facilitated through this modification? H2A.X is required for checkpoint-mediated cell cycle arrest and DNA repair following double-stranded DNA breaks (1).